Smoothing of the thermal stability of DNA duplexes by using modified nucleosides and chaotropic agents.
نویسندگان
چکیده
The effect of alkyltrimethylammonium ions on the thermostability of natural and modified DNA duplexes has been investigated. We have shown that the use of tetramethylammonium ions TMA+along with the chemical modification of duplexes allow the fine adjustment of T m and the possibility of obtaining several duplex systems with varied isostabilizedtemperatures, some of which show greater stability than those of natural DNA. This approach could be very useful for DNA sequencing by hybridization.
منابع مشابه
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 27 6 شماره
صفحات -
تاریخ انتشار 1999